06-Jan-2025

Certificate Course on Molecular Biology & Biotechnology A 100-hour taught course - 2025

To whom?

Anyone interested in learning Molecular Biology and Biotechnology, including university academics, research officers, research assistants, technical officers and field officers, graduate and undergraduate students of any discipline, candidates waiting to enter university and A/L students from any discipline.

Theory

Genetic mechanisms (DNA replication, repair, recombination, gene, genome organization, gene expression), genomes, transcriptomes, proteomes, eukaryotic nuclear genomes, genomes of prokaryotes and eukaryotic organelles, virus genomes, and mobile genetic elements.
Useful molecular biological techniques, such as DNA, RNA extraction, PCR, quantitative PCR, primer design, gel electrophoresis, genotyping techniques, bioinformatics, next generation seque

ncing techniques, sequencing genomes and understanding sequence data, RNAseq, NCBI resources, molecular phylogenetics, gene cloning, microarrays, and directed mutagenesis.

Gene regulation, gene expression, manipulation of gene expression, gene silencing (RNAi and other), gene editing, genetically modified organisms, Biosafety related topics and experimental model system.

Practical

Basic Molecular Techniques: (DNA extraction-plant, blood and microbial), Primer design, PCR, Gel electrophoresis, Demonstration on RNA extraction
Cloning Techniques: Competent cells preparation, Restriction Enzyme digestion, Ligation, Transformation, Screening of transgenic colonies
Bioinformatics: NCBI Resources & other Bioinformatics databases, File formats, Gene, Genomes sequence alignment & Phylogenetic analysis (Need to bring your own laptops. Internet facility is available)
Agrobacterium mediated gene transformation, Gene gun, Transgenic detection
Demonstration on Oxford Nanopore sequencing: Library preparation, Sequencing and data analysis
Visit to laboratory with high-end facilities designed for GMO detection & Identification