11-Jan-2023

FAuRS 2021/2022 - 1st Runner-up of the Poster Presentation in the session on “ Technological Interventions & Applications in Agriculture” -Mr. A.P.C. Nuwan (AG/16/116)

Title: Bioinformatic Analysis of CRISPR-Cas System in Enterobacteriaceae 

CRISPR-Cas System is anadaptive immune system which is present in most archaea and bacteria. I selected four strains of Enterobacteriaceae as one Enterobacter cloacae and three Enterobacter hormaechei strains were used for my study. Using four  bioinformatic tools such as CRISPRCasFinder, CRISPROne,CRISPR Recognition Tool and Piler-Cr, CRISPR sites in thsese selected strains were identified. The types which those CRISPR-Cas systems are belonging to and their locations were detected. Then primers were designed for detected CRISPR arrays using Ptimer3Plus tool. Then a workflow was designed using KNIME software to analyze CRISPR-Cas data of this bacteria family.

Conclusions 

All selected strains consisted of CRISPR-Cas systems and the

y were belonging to type I-A and IV-A systems.

CRISPRCasFinder can be recommended as an accurate tool to detect CRISPR arrays while CRISPROne can be recommended to detect Cas genes present in a genome. The KNIME Software is an ideal tool to design workflows to obtain desired outputs.